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Whey Acidic Protein [id=WAP0001]

Producer Organism : Native Protein : Production Method :
Rat Whey Acidic Protein LC method and SDS page and Immunoblotting
Activity : Antibacterial
Target Organisms :

Gram-positive: Active against Staphylococcus aureus JCM 2413 (IC50=7 µM).

NOTE: No activity against Staphylococcus aureus MRSA10, Escherichia coli JCM5491, Escherichia coli ML35P (no MIC) .

Description :
Production method: Purification from milk.

The whey acidic protein with a four-disulfide core structure (WAP motif) composed of eight cysteine residues has been identified in milk in a range of species. This is the first case report of the antimicrobial activity of WAP (Citation 1).
Citation: 1

Bacteriostatic Activity of Whey Acidic Protein (WAP)

Cited Entries: WAP0001

Authors:Iwamori, T., Nukumi, N., Itoh, K., Kano, K., Naito, K., Kurohmaru, M., Yamanouchi, K., Tojo, H.
Journal: The journal of veterinary medical science 2010, 72(5).
Abstract: We have previously reported the action of whey acidic protein (WAP) inhibiting the proliferation of mouse mammary epithelial cells in the experiments utilizing in vivo and in vitro systems. We report herein the bacteriostatic activity of WAP. Western blot analysis demonstrated successful isolation of WAP from whey fractions of rat milk by column chromatography. The WAP fraction inhibited the growth of Staphylococcus aureus JCM2413 in a dose-dependent manner, but did not inhibit the growth of Escherichia coli. The bacteriostatic activity of WAP was highest at pH 6.6 and was not affected by the presence of 150 mM NaCl. A scanning electron micrograph of bacteria treated with WAP exhibited the disruption of the bacterial cell walls.
Keywords: antimicrobial peptides, antimicrobial resistance, antimicrobial substance.

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