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LF f(17-31) modified [id=LFB0180]

Synonym: LFB Tpc 6

Producer Organism : Native Protein : Production Method :
Cow Lactoferrin (LF) Synthetic
Activity : Antibacterial
Target Organisms :

Gram-positive: Staphylococcus aureus ATCC 25923 (MIC=4.3 µM).

Gram-negative: Escherichia coli ATCC 25922 (MIC=3.2 µM).

Description :
Production method: Synthetic.

Sixth residue replaced with amino acid Tpc.
Length : 15 Mass (Da): 1 764.57 Common Amino Acids : KRA
Isolectric Point : 12.26 Net Charge : 6 Absent Amino Acids : DEHINPSTVWY
Basic Residues : 6 Acidic Residues : 0 Hydrophobic Residues : 5
Polar Residues : 2 Boman Index : -49.05 Hydropathy Index : -0.847
Aliphatic Index : 46 Instability Index : 0 Extinction Coefficient : 0
Absorbance 280nm : 0

Wheel representation

Hydrophobicity plot

Red solid plot : values according to the hydrophobicity scale of Kyte and Doolittle (reference paper).
Yellow dashed plot : Experimentally determined hydrophobicity scale for proteins at membrane interfaces(reference paper).
Green dotted-dashed plot : prediction of transmembrane helices (reference paper). In this scale (unlike the others), more negative values reflect greater hydrophobicity.

Citation: 1

Synthesis of a 2-arylsulphonylated tryptophan: the antibacterial activity of bovine lactoferricin peptides containing Trp(2-Pmc)

Cited Entries: LFB0180, LFB0181, LFB0182

Authors:Haug, B. E., Andersen, J., Rekdal, Ø, Svendsen, J. S.
Journal: Journal of Peptide Science 2002, 8(7): 7.
Abstract: A modified tryptophan, β-[2-(2,2,5,7,8-pentamethylchroman-6-sulphonyl)-indol-3-yl]alanine, Trp(2 − Pmc) = Tpc has been synthesized. Replacement of tryptophan in a bovine lactoferricin model peptide with the modified tryptophan resulted in peptides with a substantially increased antibacterial activity against Escherichia coli and Staphylococcus aureus. The most active peptides against each bacterial strain displayed minimal inhibitory concentrations of 7.5 μg/ml.
Keywords: tryptophan;side-reaction of tryptophan;Pmc;antibacterial peptide;bovine lactoferricin

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